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This study was conducted to assess the effect of the leucine metabolite, 3-hydroxy-3-methylbutyrate (HMB) on animal performance, and also cathepsins and calpain II activities in the gastrocnemius muscle in young rats undergoing dexamethasone (DX) treatment and subsequent recovery. Five days of DX administration resulted in an increase in calpain activity. During 5 days of recovery alone, calpain activity...
The purpose of this study was to determine whether inflammatory changes in uterus caused by Escherichia coli are associated with changes in nitric oxide (NO) production. Therefore, the activity of NADPH-diaphorase (NADPH-d), a histochemical marker for nitric oxide synthase (NOS), localization of inducible isoform of NOS (iNOS) and tissue content of nitrite were studied in the uterine structures after...
The expression of metallothionein (MT) was investigated immunohistochemically in the cells of total blood smears of cows infected with bovine leukemia virus, smears from in vitro culture of1 leukemic lymphocyte and in histological preparations of leukemic affected lymph nodes. In the blood smears cells and in -vitrtréulture lymphocytes, MT, was localized under cellular membrane in the form of fine...
A cannula was inserted into the aorta abdominalis through the coccygeal artery (cranial to the origin of the ovarian artery) in mature heifers (n=4) and in old cows (n=4) with reproductive disorders, to facilitate infusion of noradrenaline (NA; 4 mg/30 min) on day 10 of the oestrous cycle. Before, during and after NA jnfusion peripheral blood samples were collected for progesterone and oxytocin determination...
The studies in vitro were carried out on isolated thymic cells of Balb/c mice. The thymocyte cultures were incubated with calf thymus extract (TFX) and diethyldithiocarbamate (DTC) at a concentrations of 0.1-100 μg/ml or zinc ions (as sulphate salt) at a concentration of 1 μg/ml, and next the latter culture was supplemented with hydrocortisone at a concentration of 50 μg/ml/culture. The results of...
Studies were performed on the capacity of thrombocytes and of neutrophilic granulocytes in carp to ingest the standard bacterial strain of Staphylococcus aureus 209P Oxford. The bactericidal capacity of the cells at various developmental stages of the carp and in relation to their health status was also studied. The studies were performed on healthy carp, 3 months to 5 years of age, and sick carp,...
We previously demonstrated that both mitogenicity and anabolism in rat L6 myoblasts even in the presence of insulin could be markedly reduced by milimolar concentrations of H202, SIN-1 (3-mor- pholinosydnonimine) and SNP (sodium nitroprusside) - donors of peroxynitrite (ONOO ) and nitric oxide (NO), respectively (Orzechowski and Grzelkowska 2000). In this paper we described the results of the experiment,...
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